DEAE cellulose
Definition
- Noun:
- A type of modified cellulose used as an ion-exchange material in chromatography: DEAE cellulose is a specific chromatographic medium. It is a derivative of cellulose where diethylaminoethyl (DEAE) groups are attached. These groups give it anion-exchange properties, making it useful for separating and purifying molecules based on their charge, particularly in biochemical and analytical applications.
Usage Examples
- Noun:
- The protein mixture was purified using a column packed with DEAE cellulose.
- DEAE cellulose is a common stationary phase for the chromatography of nucleic acids.
Advanced Usage
- "DEAE-cellulose chromatography": A specific chromatographic technique that uses DEAE cellulose as the solid phase.
- They employed DEAE-cellulose chromatography to isolate the enzyme from the crude extract.
Variants and Related Words
- DEAE-Sephadex (n): A similar ion-exchange medium where the DEAE groups are attached to a Sephadex (cross-linked dextran) matrix instead of cellulose.
- For larger scale purification, DEAE-Sephadex may be preferred.
- Anion exchanger (n): A general term for a substance, like DEAE cellulose, that can exchange negatively charged ions.
- DEAE cellulose is a weak anion exchanger.
Synonyms
- Diethylaminoethyl cellulose: The full chemical name for DEAE cellulose.
- Anion-exchange cellulose: A descriptive term highlighting its function and base material.
Noun
-
used for chromatography
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